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Characterization of aptamer YQ26. (A) Secondary structure of aptamer YQ26 predicted by NUPACK. (B) Dissociation constant of aptamer YQ26 for mEND-HEK293 cells. (C) Binding of aptamer YQ26 to trypsin-treated mEND-HEK293 cells. (D) Binding of aptamer YQ26 to mEND-HEK293 cells at 4°C and 37°C. The concentration of DNA sequences was 250 nM in all assays.
