Table 1. Primers used in this study.
Primer name | Sequence(5’-3’) | Purpose |
---|---|---|
eae F | tgcggcacaacaggcggcga | Gene cloning |
eae R | cggtcgccgcaccaggattc | |
Stx1B F | cccggatccatgaaaaaaacattattaatagc | Gene cloning |
Stx1B R | cccgaattcagctattctgagtcaacg | |
Stx2A and variants F | atcctattcccgggagtttacg | Gene cloning |
Stx2A and variants R | gcgtcatcgtatacacaggagc | |
hlyA F | ggtgcagcagaaaaagttgtag | Gene cloning |
hlyA R | tctcgcctgatagtgtttggta | |
IL-1β F | tggcacgtatgagctgaaag | RT-PCR |
IL-1β R | ggccacaggtatcttgtcgt | |
IL-4 F | cactccggcagttctacctc | RT-PCR |
IL-4 R | gcagaggttcctgtcgagtc | |
IL-6 F | ctgaagacgaccacgatcca | RT-PCR |
IL-6 R | aaggacacccgcactccat | |
IL-8 F | ctctcttggcaaccttcctg | RT-PCR |
IL-8 R | ttgcacagtgaggtccactc | |
IL-10 F | aaaagctaaaagccccagga | RT-PCR |
IL-10 R | cgggagctgaggtatcagag | |
IFN-γ F | ctcgaatttcggtggatgat | RT-PCR |
IFN-γ R | agcgtctgactcctttttcg | |
TNF-α F | cacttcagggtgatcggc | RT-PCR |
TNF-α R | tgcgggtttgctactacg | |
DEFB114 F | taccagccacatgctctttg | RT-PCR |
DEFB114 R | cctgtcgacacagcaaatct | |
DEFB124 F | gcaccaagcaagagtccttc | RT-PCR |
DEFB124 R | acgccagagccagctactta | |
DEFB125 F | cgtgctgcatctccttaaca | RT-PCR |
DEFB125 R | gcgaagcagaaaattgatcc | |
DEFB127 F | cccacagtaaccgagcaact | RT-PCR |
DEFB127 R | gctgaggcagcagtatctcc | |
DEFB128 F | gggctcaaggctttctcttt | RT-PCR |
DEFB128 R | aaatctcgcctagcttgcac | |
DEFB134 F | agcctgtctgcctggagtag | RT-PCR |
DEFB134 R | gatgaggagaggcttcatgg | |
DEFB135 F | gctgcatctccaaatccaat | RT-PCR |
DEFB155 R | tagtgggatggtgcaactga | |
NP 5 F | aggcaggcgtgttctgtact | RT-PCR |
NP 5 R | ggtctccacgcaaataagga | |
GAPDH F | aggtcatccacgaccacttc | RT-PCR |
GAPDH R | gtgagtttcccgttcagctc |