Table 5. Stratified analyses between PADI4 rs11203366 A>G polymorphism and ESCC risk by sex, age, smoking status and alcohol consumption.
| Variable | rs11203366 A>G (case/control) a | Adjusted OR b (95%CI); p; phc | |||||||
|---|---|---|---|---|---|---|---|---|---|
| GG | AG | AA | AG+AA | GG | AG | AA | AG+AA | AA vs. (GG+AG) | |
| Sex | |||||||||
| Male | 68/83 | 209/203 | 157/152 | 366/355 | 1.00 | 1.26(0.86-1.83); p:0.254 ; ph:0.304 | 1.26(0.85-1.86); p: 0.275; ph:0.879 |
1.26 (0.89-1.79); p: 0.211; ph:0.706 |
1.06 (0.81-1.41); p:0.67; ph:0.102 |
| Female | 35/55 | 84/98 | 62/62 | 146/160 | 1.00 | 1.35 (0.81-2.25); p:0.299 ; ph:0.304 |
1.57 (0.91-2.73); p:0.126 ; ph:0.879 |
1.43(0.89-2.32); p: 0.150; ph:0.706 |
0.78 (0.51-1.19); p:0.277 ; ph:0.102 |
| Age | |||||||||
| <63 | 51/82 | 136/147 | 114/112 | 250/259 | 1.00 | 1.49(0.98-2.26); p:0.073 ; ph:0.555 |
1.64(1.06-2.53); p:0.029 ; ph:0.953 |
1.56(1.05-2.29); p: 0.032; ph:0.676 |
0.80 (0.58-1.11); p:0.19; ph:0.102 |
| ≥63 | 52/56 | 157/154 | 105/102 | 262/256 | 1.00 | 1.09(0.71-1.70); p:0.740 ; ph:0.555 |
1.11(0.69-1.76); p:0.720 ; ph:0.953 |
1.10(0.73-1.67); p: 0.670; ph:0.676 |
0.97(0.69-1.35); p:0.865 ; ph:0.102 |
| Smoking status | |||||||||
| Never | 60/110 | 173/220 | 111/145 | 284/365 | 1.00 | 0.69(0.48-1.01); p:0.062 ; ph:0.000 |
0.71(0.48-1.06); p:0.713 ; ph:0.000 |
0.70(0.49-0.99); p: 0.055; ph:0.978 |
1.08(0.80-1.46); p:0.59 ; ph:0.124 |
| Ever | 43/28 | 120/81 | 108/69 | 228/150 | 1.00 | 1.04(0.59-1.8); p:1.000 ; ph:0.000 |
0.98(0.56-1.72); p:1.000; ph:0.000 |
1.01(0.60-1.69); p:1.000; ph:0.978 |
1.05(0.71-1.54); p:0.844; ph:0.124 |
| Alcohol consumption | |||||||||
| Never | 73/119 | 198/231 | 144/151 | 342/382 | 1.00 | 0.66(0.46-0.94); p:0.066; ph:0.013 |
0.64(0.44-0.93); p:0.020; ph:0.283 |
0.69(0.50-0.95); p:0.023; ph:0.778 |
0.50(0.37-0.68); p:0.155; ph:0.146 |
| Ever | 30/19 | 95/70 | 75/63 | 170/133 | 1.00 | 1.16(0.61-2.23); p:0.742; ph:0.013 |
1.33(0.68-2.58); p:0.503; ph:0.283 |
1.24(0.67-2.29); p:0.537; ph:0.778 |
0.85(0.55-1.30); p:0.509; ph:0.146 |
a The genotyping success rate was 96.42% for rs11203366 A>G; b Adjusted for age, sex, smoking status and alcohol consumption (besides stratified factors accordingly) in a logistic regression model; c ph for heterogeneity; Bold values are statistically significant (p<0.05).
PADI4 rs11203366 variant AA was associated with ESCC among younger patients (<63 years) (p=0.029). In the dominant model, PADI4 rs11203366 was associated with ESCC among younger patients (<63 years) (p=0.032). In the cohort of subjects who carry PADI4 rs11203366 AG variant or AA variant, smoking significantly increased the ESCC susceptibility (ph=0.000).
In the non-alcohol drinking cohort, PADI4 rs11203366 AA (p=0.020) variant was associated with increased risk of ESCC.
In the dominant (p=0.023) model, PADI4 rs11203366 A>G was associated with increased risk of ESCC.
In the PADI4 rs11203366 AG subgroup, alcohol drinking significantly increased the risk of ESCC (ph=0.013).