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. 2017 Oct 5;8(55):93741–93756. doi: 10.18632/oncotarget.21526

Figure 3. Effects of prenatal nicotine exposure on the thymocyte phenotypes and apoptosis frequency in the fetus on GD 18.

Figure 3

Pregnant mice were sacrificed after anesthesia on GD 18. Fetuses were removed quickly from the uterus and decapitated after euthanasia. The fetal thymus from each littermate were collected and pooled into one sample for flow cytometry detection. (A) Typical flow diagram of thymocyte phenotypes; (B) Frequency of thymocyte subsets; (C) Typical flow diagram of thymocyte apoptosis; (D) Apoptosis frequency of thymocyte. The difference was analyzed with t-test. Mean ± SD, n = 3–4. *P < 0.05 vs control.