Skip to main content
. 2017 Sep 8;8(55):93757–93770. doi: 10.18632/oncotarget.20771

Figure 3. Knockdown of LC3 inhibited both autophagic capacity and the expression of Bcl-xL in HSFs.

Figure 3

HSFs, with 50-70% confluent, were infected by AdLC3, shLC3 and their negative control adenovirus vectors, cultured in DMEM medium for 36-48 h, and then analyzed with Western blot. (A, B) The ratio of LC3-II/LC3-L changes in infected HSFs. (A, C) auophagy protein Beclin 1 expresses and changes in the Beclin 1/β-actin ratio. (A, D) Autophagic flux protein p62 expresses and changes in the p62/β-actin ratio.(A, E) Bcl-xL protein expresses and changes in the Bcl-xL/β-actin ratio.(A, F) Bcl-2 protein expresses and changes in the Bcl-2/β-actin ratio. Data are representative of three experiments. n = 3, ▲p > 0.05, *p < 0.05, **p < 0.01 compared with the negative control group.