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. 2017 Oct 10;8(55):94358–94370. doi: 10.18632/oncotarget.21719

Figure 5. RKIP is a target gene of miR-23a and is regulated by XIST.

Figure 5

(A) Predicted miR-23a binding sites in the 3′UTR of RKIP (RKIP-Wt) and mutant sequence (RKIP-Mut) was shown. (B, C) Luciferase reporter assays were performed to determine luciferase activity in DU145 and LNCAP cells co-transfected with miR-23a mimics and luciferase reporters containing 3′UTR sequence of RKIP-Wt or RKIP-Mut. *P<0.05 vs. miR-23a NC group. (D, E) Relative expression of RKIP was examined by western blot in DU145 and LNCAP cells transfected with miR-23a mimics and inhibitors. *P<0.05 vs. miR-NC group. (F, G) Relative expression of RKIP in DU145 cells transfected with XIST or NC and LNCAP cells transfected with si-XIST or si-NC. *P<0.05 vs. NC group or si-NC group. (H) Relative expression of RKIP in DU145 cells co-transfected with XIST and miR-23a mimics or miR-23a inhibitors. *P<0.05 vs. NC group. (I) Relative expression of RKIP in LNCAP cells co-transfected with si-XIST and miR-23a or miR-23a inhibitors. *P<0.05 vs. si-NC group. Data are presented as mean ± SD from three separated experiments.