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. 2017 Aug 24;8(56):95256–95269. doi: 10.18632/oncotarget.20438

Figure 1. TCTN2 is overexpressed in primary tumors of colon lung and ovary.

Figure 1

(A) TCTN2 is overexpressed in tumor samples and localizes at the cell membrane. Cancerous and matched normal samples from colon, lung and ovary were stained with the anti-TCTN2 monoclonal antibody, arrayed in parallel on the same TMA slides and analyzed simultaneously. (B) Specific recognition of TCTN2 antibodies - TCTN2 expressed in mammalian cells is a glycosylated protein. Western blot on total protein extracts from HeLa cells transfected with the empty plasmid or full length TCTN2, or recombinant TCTN2 domain (amino acids 171-444) expressed in E. coli, using the polyclonal (pAb291-YOM, left panel) and monoclonal antibody (anti-TCTN2 mAb, right panel). The recognition of the mAb anti TCTN2 was also assessed after treatment with Peptide-N-Glycosidase F (PNGaseF).