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. 2017 Aug 24;8(56):95346–95360. doi: 10.18632/oncotarget.20524

Figure 3. Effects of overexpression and knockdown of Botch on the protein levels of Botch and the maturation of Notch1 in cultured neurons.

Figure 3

(A) Transfection efficiency of Botch expression plasmid and siRNA in cultured neurons under normal condition. (B, C) Transfection efficiency of Botch expression plasmid and siRNA in cultured neurons exposed to OxyHb. (D, E) Effects of overexpression and knockdown of Botch on the protein levels of Notch1 in neurons under OxyHb insults. In (A-E), mean values for normal group were normalized to 1.0. (A) ##p = 0.0022 vs. vector; && p = 0.0024 vs. ctr-siRNA. (B) **p = 0.0090 vs normal; #p = 0.0109 vs OxyHb + vector. (C) **p = 0.0011 vs normal; N.S. indicates no significant difference; ##p = 0.0015 vs OxyHb + ctr-siRNA. (D) **p = 0.0019 vs normal; ##p = 0.0032 vs OxyHb + vector. (E) **p = 0.0017 vs normal; ##p=0.0021 vs OxyHb + ctr-siRNA.