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Western blot analysis of sera from humans working as butchers or in the meat industry using the R1 and R2 recombinant protein of glycoprotein B (gB) of PCMV. Anti-His serum was used as a positive control (lane 2). Anti-His serum was used at a dilution of 1:1000, human sera at 1:300, and anti-human serum at 1:1000. Lane 1, marker proteins, lane 2, antibodies against the His tag, lane 4 to 14, different human sera samples.
