Table A1.
List of primers used in the study.
| Primer | Sequence 1 |
|---|---|
| 2μ ori/F | agatctcagtaaagcGAAAAGTGCCACCTGAACGA |
| TRP/R | gggggttcagccagcAAGGATCTGGCGTAATAGCG |
| eGFP/SalI/F | GCGTCGACATGGTGAGCAAGGGCGAGGA |
| eGFP/SacI/R | GCGAGCTCTTACTTGTACAGCTCGTCCAT |
| Backbone/LB/R | AAAACCACCCCAGTACATTAAAAAC |
| Backbone/RB/F | GAATTCAGATTGTCGTTTCCCG |
| 35S-p19/F | CGGACGTTTTTAATGTACTGGGGTGGTTTTACGCGTCATGGTGGAGCACG |
| NOS-p19/R | GTCTAGAGAATCTCCTGCAATCATTCCGGAAACACTGATAGTTTAATTCCCGAT |
| 35S-HCPro/F | GGAGGGACTGTATTCATGTTAAATGATAAGACGCGTCATGGTGGAGCACG |
| NOS-HCPro/R | ACTGAAGGCGGGAAACGACAATCTGAATTCAACACTGATAGTTTAATTCCCGAT |
| 35S-γb/F | TCCGGAATGATTGCAGGAGATTCTCTAGACACGCGTCATGGTGGAGCACG |
| NOS-γb/R | CTTATCATTTAACATGAATACAGTCCCTCCAACACTGATAGTTTAATTCCCGAT |
| oligo(dT)17 | GACTCGAGTCGACATCGATTTTTTTTTTTTTTTTT |
| PVY backbone/R | GTATTGAGTTGTTTTAATTTCCTCTCCAAATGAAATGAACTTCCT |
| PVY backbone/F | GATGTCGACTCGAGTCGAATTTCCCCGATCGTTCAAACATTTG |
| PVY a/F | CATTTCATTTGGAGAGGAAATTAAAACAACTCAATACAACATAAG |
| PVY a/R | TATTGCCTGACACACTGCCA |
| PVY b/F | ATCTTTAGGCGTTTGCCAAC |
| PVY b/R | ACAGGGAAATCCTTTGGCATC |
| PVY c/F | TTTTACCGATCAAAGGCAGAGAC |
| PVY c/R | TTGAACGATCGGGGAAATTCGACTCGAGTCGACATCGATT |
| PVY/b2/F | TTTGTTGATATGCAGGTGTTAAAAATTTGGAACAAGTGGT |
| PVY/b1/R | GAAGCAGAAACTTACCTGGTGTGGAACGCTGGTGT |
| PVY/b2/R | TAAGTGCATACTTACCTCTCTTCCCACTGGAGTAGCT |
| PVY/b3/F | TTTGAAATTATGCAGGTTGAATTTACAACACAGCAACCA |
| Intron1/modified/R | CTGCATATCAACAAATTTTGGTCATATATTAGAAAAGTTATAAATTAAAAT |
| Intron1/F | AGCGTTCCACACCAGGTAAGTTTCTGCTTCTACCTTTGAT |
| Intron1/R | CCAAATTTTTAACACCTGCATATCAACAAATTTTGGTCAT |
| Intron2/F | CCAGTGGGAAGAGAGGTAAGTATGCACTTAAAGAGTATGTGT |
| Intron2/R | TGTTGTAAATTCAACCTGCATAATTTCAAAGATTGAACCTA |
| SYNV backbone/F | TGTTCTGAGCTTTTGTCTCTGGGTCGGCATGGCATCTCCA |
| SYNV backbone/R | TTTTCTGAGTTTCTGTCTCTCCTCTCCAAATGAAATGAACTTC |
| SYNV a/F | GTTCATTTCATTTGGAGAGGAGAGACAGAAACTCAGAAAATACAAT |
| SYNV a/R | CACATCCTCAAGAAACATGCT |
| SYNV b/F | CCATTTGCCATGATCAGACG |
| SYNV b/R | GATGGGTCGTTTGATCGATG |
| SYNV c/F | GCTTCTGAACGACATCTGAATC |
| SYNV c/R | CCGATAGATCTCGCAAATATTGAT |
| SYNV d/F | CAAAGCAGAGGCCGTAATGAG |
| SYNV d/R | TGGAGATGCCATGCCGACCCAGAGACAAAAGCTCAGAACAAT |
| PVY/NIb/BglII/20/F | tcacaacatttctc agatcTTGGTTTG |
| PVY/NIb/eGFP15/R | gcccttgctcaccatTGCTTGATGGTGTACTTCATAAGTATCGC |
| PVY/CP/eGFP15/F | gacgagctgtacaagACTTATGAAGTACACCATCAAGCAAAT |
| PCB/BglII/15/R | cgctttactg agatcTCCTGTG |
| eGFP/F | ATGGTGAGCAAGGGCGAGGA |
| eGFP/R | TTACTTGTACAGCTCGTCCATGCCGA |
1 The sequence shown in lower letters is homologous to other primer to facilitate In-Fusion cloning; the overlapped restriction site is in italics. Underlined sequence is homologous to other primer to facilitate homologous recombination in yeast.