Figure 1.
Representative kinetic analysis of the interaction of S195A GD-FXa with immobilized tissue factor pathway inhibitor (TFPI). The GD-FXa samples were injected at the indicated concentrations over immobilized TFPI (1300 RU) in 18 mM Hepes, 135 mM NaCl, 2.5 mM CaCl2, 0.005% surfactant P20, pH 7.35. Binding is expressed in resonance units (RU). Fits are shown as red lines and were obtained by global fitting of the data using a 1:1 Langmuir binding model. The KD was calculated from 3 independent experiments.