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. 2017 Oct 10;8(53):90662–90673. doi: 10.18632/oncotarget.21730

Figure 3. CDK1 activity is required to promote nascent DNA synthesis.

Figure 3

A. Representative FACS data from U2OS treated with the indicated inhibitors for 40 minutes and then pulse-labeled with EdU in the last 10 minutes of the incubation period. EdU-positive cells were gated as shown by the black line. The experimental scheme is shown at the top. B.-C. Quantification of EdU-positive cells gated from A. D. Workflow to determine the effects of CDK1i on DNA replication in different stages of S phase. E. Quantification of mean fluorescence intensity (MFI) in EdU-positive cells after the treatments applied according to the scheme shown in D. Data in B. and C. are pooled from three independent experiments and are presented as the means±SEM. Two independent experiments were conducted for B. and data are presented as the mean±SEM. A Student’s t-test was performed for B. and B. to determine significance. Multiple t-tests were performed for E. *, p < 0.05; **, p < 0.01, ****, p < 0.0001; ns, not significant.