Figure 6. Increased basal levels of apoptotic thymocytes and reduced total thymocyte number in TRPV1 KO mice.

Freshly purified thymocytes from WT and KO mice were counted using trypan blue solution A., or stained with Annexin-V FITC and analyzed by FACS analysis B. Data are the mean ± SEM of three separate experiments, *p < 0.01 vs WT thymocytes. C. From the total number of cells per thymus, the relative number of cells in each CD4/CD8 subset was determined staining TRPV1 KO and WT thymocytes with anti-CD4-PE and anti-CD8-Cy5 mAbs and using FACS analysis. Data are the mean ± SEM of three separate experiments *p < 0.01 TRPV1 KO vs WT thymocytes.