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. 2017 Jan 24;8(7):1170–1188. doi: 10.1080/21505594.2016.1278333

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Verification of knockout strains. (A) Genomic DNA of indicated strains was isolated and the deletion of target genes was verified by PCR. The expected product sizes are indicated in the table on the right-hand side. (B) Total RNA was extracted from Ye wildtype and the different mutant strains. The deletion of target genes was verified by qRT-PCR using gyrA as reference gene. (C) Ye whole cell lysates were probed with antibodies raised against E. coli BamB or BamC that exhibited cross-reactivity with the respective Ye proteins (all other available antibodies did not cross-react with the Ye homologs). The calculated molecular weight of the tested proteins from E. coli and Ye homologs are indicated in the table on the right-hand side.