Figure 3.

Effects of miRNAs and miRNA target genes on Edwardsiella tarda infection. (A) Tongue sole were infected with E. tarda in the presence of the agomir of each of the downregulated miRNAs, or agomir plus the plasmid that overexpresses the target gene of the miRNA. Bacterial numbers in spleen were determined at 48 h post-infection (hpi). (B) Tongue sole were infected with E. tarda in the presence of the antagomir of each of the upregulated miRNAs, or antagomir plus the siRNA against the target genes of the miRNAs. Bacterial numbers in spleen were determined as above. The experiments were performed 3 times, and values are shown as means ± SEM. *P < 0.05, **P < 0.01.