Scheme 2.

Proposed PT reactions associated with ligand dissociation from [RNA+Gpa−4 H]⁴⁻ ions with energy requirements of A) E ₁, B) E ₂, and C) E ₃, for which E ₁<E ₂<E ₃. At elevated energy E ₃, changes in the higher‐order RNA structure allow for PT from an RNA phosphodiester moiety (shown in violet) that was not initially bound to Gpa. Other RNA–ligand interactions that potentially stabilize the complex structures before and after PT are omitted for clarity.