Figure 2. Large proportions of accessibility changes occur at TSS sites and accessibility changes are seen dependent and independent of DNA methylation during EMT.

(A) Quantification of probes that get increased accessibility and decreased accessibility at any time point > 0.3 delta-beta in different genomic regions. (B) Pie charts depicting the occurrence of gain and loss of accessibility changes with respect to genomic context for TWIST-induced EMT shows that a large proportion of changes localize to the TSS. (C) Smooth scatter plots of delta-methylation (methylation in treated – methylation in control) and delta-accessibility (accessibility in treated – accessibility in control) for day 2 in TWIST-overexpressing cells. (D and E) GSEA of gain and loss of accessibility genes