Fig. 3.

A poorly conserved 774-bp element is sufficient to drive expression within the NPCs. (A) Schematic of three different transgenic constructs used to generate transgenic lines. Red ovals represent consensus Lef/Tcf-binding sites. (B) Sections of stained kidneys from embryos injected with each construct whose DNA was scored as positive for carrying the lacZ transgene. The right-hand image shows a P1 Fam19a5-GRE-2161-2934 section stained with β-gal (bgal, green), Six2 (red) and DAPI (blue). Arrows indicate the nephron progenitor cells. Scale bars: 50 μm.