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. 2017 Nov 29;20(1):57–68. doi: 10.1016/j.neo.2017.10.007

Figure 2.

Figure 2

Overexpression of ISG20 significantly promotes tumor angiogenesis in vivo. (A) Mice were subcutaneously injected with Matrigel mixed with control or ISG20-overexpressing cells (SK-Hep1 and J7) for 7 days. Plugs excised from mice were photographed (n = 8). (B) ISG20-overexpressing and control cells mixed in Matrigel were placed on chick CAMs, and images of CAM artery branches in groups obtained on developmental day 12 (left panel). Quantification of the microvessel number in the CAM assay (n = 3, right panel). (C) Hemoglobin content in plugs isolated from mice at day 7, measured using Drabkin’s procedure. Tumor (plug) volume and weight were measured. (D) Levels of ISG20 and the endothelial marker, CD31, were determined from plugs of ISG20-overexpressing and control cells via IHC staining. Data are presented as means ± SD (*p < 0.05; **p < 0.01).