Table 1B.
Sequences of primers used for mMyoD, hMyoD, hCK-M, hMyogenin, hMHC, hDystrophin, hMyomaker.
| Gene name | Number cycle | Ann. temp. | Sequence |
|---|---|---|---|
| hβactin | 30 | 55 | CTCTTCCAGCCTTCCTTCCT |
| CACCTTCACCGTTCCAGTTT | |||
| mMyoD | 30 | 55 | CTTCTATGACCCGTGTTTCGAC |
| CTGGGTTCCCTGTTCTGTGT | |||
| hMyoD | 30 | 61 | CACTCCGGTCCCAAATGTAG |
| TTCCCTGTAGCACCACACAC | |||
| hCK-M | 30 | 55 | ACATGGCCAAGGTACTGACC |
| TGATGGGGTCAAAGAGTTCC | |||
| hMyogenin | 30 | 64 | TAAGGTGTGTAAGGGAAGTCG |
| CCACAGACACATCTTCCACTGT | |||
| hMHC | 30 | 61 | CTGCTGAAGGAGAGGGAGCT |
| TGATTAGCTGGTCACACCTT | |||
| hDystrophin | 30 | 61 | GATGCACGAATGGATGACAC |
| TGTGCTACAGGTGGAGCTTG | |||
| hMyomaker | 30 | 61 | GAAGGAGAAGAAGGGCCTGT |
| CCTTCTTGTTGACCTTGGGC |