Skip to main content
. 2017 Oct 23;8(57):97313–97322. doi: 10.18632/oncotarget.21960

Figure 6. MiR-204 enhances mitochondrial apoptosis in DOX-treated PCa cells.

Figure 6

(A) Effect of DOX (0.5 μM), miR-204 and SIRT1 plasmid on changing the expression levels of Puma and Noxa in C4-2 and LNCaP cells. (B) After removal of mitochondria, cyto c in cytoplasm of C4-2 and LNCaP cells was detected by using western blot analysis. (C) Interaction with Apaf-1 and casp.9 was tested by co-immunoprecipitation assays in C4-2 and LNCaP cells treated with DOX (0.5 μM), miR-204 and SIRT1 plasmid. (D) Effect of DOX (0.5 μM), miR-204 and SIRT1 plasmid on changing the cleavage of casp.9, casp.3 and PARP. (E) Apoptotic rate of C4-2 and LNCaP cells was measured after they were treated with DOX (0.5 μM), miR-204 and SIRT1 plasmid. *P<0.05 vs. DOX + NCO group, #P<0.05 vs. DOX + miR-204 group.