Figure 6.

(A) Immunohistochemical staining for HMGA2 protein in normal, well- and poorly differnetiated PDAC (HMGA2 antibody, hematoxylin counterstain) with lower magnification images (5×, left) and its expanded views (20×, right). (B, C) Wound healing assay. PANC-1 cell was transfected with Negative Control (NC) and si-HMGA2. Optical images of wounded cell monolayers were taken after 0 h, 24 h and 48h. The remaining distance was calculated as a percentage of the initial wound area. The data presented were mean ± SEM of three independent experiments performed separately; ^*P<0.05 or ^**P<0.01. (D, E) PANC1 cells transfected NC and si-HMGA2 cells were induced to invade through Matrigel-coated transwell membranes. The number of migrated cells was counted. ^** P < 0.01.