Figure 4.

Stable gene silencing reduced the adhesion and motility of ND‐01. (a) Adhesion capacity of stably silenced ND‐01 to mucus. Data are presented as the means ± SD. Three independent biological replicates were included for each group. **p < .01 compared with the control. (b) Diameter of the colony of each strain. Data are presented as the means ± SD., and three independent biological replicates were included for each group. The means of treatments not sharing a common letter are significantly different at p < .05. (c–g) Typical images of spreading of control (c) and mcp‐ (d), aer‐ (e), cheV‐ (f), cheB‐ (g) and flgA‐ (h) silenced ND‐01 cells