Table 1.
Strains used in this study
| Strains | Relevant characteristics | Reference |
|---|---|---|
| Plasmid | ||
| pUX‐BF13 | RK6 replicon – based helper plasmid; Apr | Choi et al. 2006 |
| pEX100T | oriT, bla, sacB | Michelsen et al. 2015 |
| pEX100T::nunF | oriT, bla, sacB, nunF and arms for HR*, Apr | This study |
| pEX100T::nunF::Gm r | oriT, bla, sacB, nunF, GmR, Ampr | This study |
| pHN1270 | broad ‐ host range plasmid, oriRK2, Aprr | Nakashima & Tamura, 2004 |
| pHN1270::nunF | complementation of nunF | This study |
| pSEVA237R | broad ‐ host range plasmid, oriBBR1, Kmr | Silva‐Rocha et al. 2013 |
| pSEVA237R::PnunF | nunF promoter region fused to mCherry fluorescent protein, Kmr | This study |
| Bacterial | ||
| Eschericia coli DH5α™ | endA1 hsdR17 supE44 thi‐1 recA1 U169 deoR | NEB, UK |
| Pseudomonas fluorescens In5 | wild‐type | Michelsen et al. 2015 |
| P. fluorescens In5 | ΔnunF::Gmr | This study |
| P. fluorescens In5 | 5F5 nunE::Tn5Kmr | Michelsen et al. 2015 |
| P. fluorescens In5 | M2D1 nrps::Tn5Kmr | This study |
| P. fluorescens SS101 | wild‐type | de Bruijn et al. 2008 |
| Fungal and oomycete | ||
| Rhizoctonia solani Ag3 | wild‐type | Michelsen & Stougaard, 2011 |
| Pythium aphanidermatum | wild‐type | Michelsen & Stougaard, 2011 |
| Fusarium graminearum PH1 | wild‐type | Frandsen et al. 2006 |
| Neurospora crassa 4200 | wild‐type | Colot et al. 2006 |
* HR; homologous recombination