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. Author manuscript; available in PMC: 2017 Dec 13.
Published in final edited form as: Biochemistry. 2016 Apr 21;55(17):2427–2440. doi: 10.1021/acs.biochem.6b00102

Figure 6.

Figure 6

(A) Förster resonance energy transfer (FRET) between oxazole 1a at position 66 of GFP and Acd at position 39 of the same protein. The sample was excited at 296 nm, affording a FRET signal at ~450 nm (blue). A control sample having 1a at position 66, but no acceptor, exhibited no FRET upon being irradiated at 300 nm (Figure 4). Irradiation of the same sample at the excitation wavelength of Acd (370 nm) produced fluorescence emission centered at ~450 nm (orange). The sample was present at a concentration of ~20 nM in 25 mM Tris-HCl (pH 7.4) containing 0.25 M NaCl. (B) Model based on the X-ray crystal structure of green fluorescent protein (Protein Data Bank entry 1GFL), showing the placement of oxazole 1a in lieu of Tyr66, and Acd in place of Tyr39.