Figure 3. Viability of LHCN-M2 cells after pre-treatment with or without plant extracts for 48 h followed by exposure to hydrogen peroxide.

Cells were cultivated in presence of (A) CE (1 and 3 μg/mL), EtOAc (1 and 3 μg/mL) and VitC (1 μ g/mL) or (B) gallic acid (1, 3, 10 and 30 μ M) for 48 h prior to stimulation with 75 µM H₂O₂ for 24 h. The results were expressed as a percentage of treated cells normalized to untreated control cells. Untreated cells were used as the negative control; VitC was used as positive control. Mean values (% of control) with S.D. are indicated. This experiment was performed in duplicate and repeated three times. The significance (^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001) of cell viability in treated cells/+H₂O₂ with respect to the untreated cells/−H₂O₂.