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. Author manuscript; available in PMC: 2018 Dec 5.
Published in final edited form as: Structure. 2017 Nov 16;25(12):1839–1855.e11. doi: 10.1016/j.str.2017.10.007

Figure 6.

Figure 6

Ubistatins shield polyUb from disassembly by DUBs. (A) Disassembly of K48-Ub2 by Ubp6 over the indicated time course in the presence of varying concentrations of h-ubiB (left) or ubiB (right). (B) Linkage-selective DUBs rapidly disassemble their respective linkage type (left), while stoichiometric amounts of ubiB prevent any cleavage within the time course (right). (C) Disassembly of K11-Ub6+ by yeast 26S proteasome in the absence (left) and presence (right) of ubiB. (D) Processing of Ubch5b-Ubn substrate by the proteasome in the absence (top row) or presence (bottom row) of ubiB. The change in the Ub profile over time was followed with the indicated antibody.