Figure 1. Cell volume changes of HGC-27 cells during hypotonic stimulation.

The cell volume and counts of HGC-27 cells were simultaneously measured at 1, 5, 10 and 20 min after hypotonic stimulation with various osmolalities. The isotonic NaCl solution was diluted 2-, 4-, 8-, or 16-fold with distilled water, and shown as 1/2, 1/4, 1/8 or 1/16 NaCl solution, respectively. The cell volume in the isotonic NaCl solution was used as a sample without hypotonic stimulation (0 min).