Figure 7. TCTP was regulated by P53.

(A) Knockdown P53 together with P53 can partly reverse the effect caused by TCTP knockdown. (B) Overexpress P53 can downregulate TCTP protein level. (C) Overexpress P53 can downregulate TCTP mRNA level. (D) At the top is a schematic representation of the P53 binding sequence in the TCTP promoter; at the middle is the western results of RIP assay to prove the antibody can pulldown P53 and the mRNA it bound. Bottom is the QPCR results to testify the binding sequence. (E) Luciferase assay to test the P53 binding sequence in TCTP promoter and its mutant.P53 was either knockdown or overexpressed and check its effect on luciferase activity. Data represent the mean ± s.d. of 3 independent experiments. ^***P ≤ 0.0001 ^**P≤ 0.001.