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. 2017 Sep 21;8(61):103261–103273. doi: 10.18632/oncotarget.21143

Figure 3. Suppressive miR-129 functions through inhibition on ESR1 and NOTCH signaling in breast cancer stem cells.

Figure 3

Breast cancer stem-like cells could be identified by CD44+/24- phenotype (A), which were capable of forming much more spheres than the subtype of CD44-/24+ marker (B). (C) MCF-7 stem-like cells of CD44+/24- phenotype preserved decreased miR-129 expression, together with upregulated ESR1 level and NOTCH 1, the NOTCH signaling activation indicator. (D) Enforced miR-129 expression decreased the proportion of CD44+/24- cells significantly, however NUMB inhibition induced NOTCH activation abolished the suppressive functions of miR-129. (E) MiR-129 decreased the mammosphere number, and the siRNA of NUMB induction of NOTCH activation abolished suppressive functions of miR-129, suggesting that miR-129 functioned through maintaining NUMB expression level.