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. 2017 Oct 31;8(61):104247–104257. doi: 10.18632/oncotarget.22200

Figure 2. Metformin reversed EMT induced by bFGF in HepG2 and Huh7 cells.

Figure 2

(A) Expression of phenotypic proteins including E-cad, N-cad and Vimentin in cells treated without and with bFGF (20 μg/ml) or bFGF (20 μg/ml) and metformin (10 μmol/ml) together was analysed by western blotting. (B) Metastatic potential of cells treated without and with bFGF or bFGF and metformin together was evaluated by wound healing scratch assay. (C, D) Migration and invasion of cells treated without and with bFGF or bFGF and metformin together were assessed using transwell assay. *p<0.05 compared with the control, #p<0.05 compared with bFGF treatment.