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. 2017 Nov 6;8(61):104418–104429. doi: 10.18632/oncotarget.22301

Figure 2. Characterization of t-BMSCs, TERT-BMSCs and normal BMSCs.

Figure 2

(A) Representative images show immunofluorescence staining of CD44, CD105, CD29, CD90, CD31, CD34, CD45, and CD11b cell surface markers in TERT-BMSCs, tBMSCs, and BMSCs. As shown, all 3 BMSCs express high levels of CD44, CD105, CD29 and CD90, but do not express CD31, CD34, CD45, and CD11b (bar: 20μm). (B) Representative FACS plots show cell surface staining of CD44, CD105, CD29, CD90, CD31, CD34, CD45, and CD11b in TERT-BMSCs, tBMSCs, and BMSCs. As shown, all 3 BMSCs TERT showed high expression of CD44, CD105, CD29, and CD90, but did not express CD31, CD34, CD45, or CD11b. Therefore, tBMSCs and TERT-BMSCs retain differentiation markers of BMSCs. (C) RT-PCR (left) and western blot (right) analysis show TERT mRNA and protein expression in TERT-BMSCs, tBMSCs, and BMSCs. RAW264.7 cells were used as positive control for TERT. β-actin was used as internal control. TERT-BMSCs and tBMSCs show high expression of TERT than normal BMSCs.