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. 2018 Jan;24(1):6–11. doi: 10.1261/rna.063891.117

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© 2018 Naruse et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — In vitro reconstitution of human Ago2-RISC assembly by recombinant proteins. (A,B) Coomassie staining of immunopurified Ago2 and recombinant Hsp70/Hsp90 chaperone machinery proteins. (C) Target RNA cleavage assay by reconstituted human Ago2-RISC. The highest cleavage activity was observed when all five chaperone proteins were present. (D) Quantification of target cleavage in Figure 1C. The fraction target cleaved is the amount of cleaved target divided by the sum of full-length target and cleaved target. The mean ± SD from three independent experiments is shown.