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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Nature. 2017 Mar 29;544(7650):316–321. doi: 10.1038/nature21720

Extended Data Figure 5. Double deletion of both Syt3 and Syt5 (Syt3,5 DKO) does not alter LTP and BDNF does not rescue the blocked LTP in Syt1,7 double-deficient neurons.

Extended Data Figure 5

a, Field EPSPs (fEPSPs) recordings from heterozygous (Syt3,5 Het) and homozygous constitutive Syt3 and Syt5 double KO mice (Syt3,5 DKO). Left, representative traces and LTP time course; center and right, cumulative frequency plots and summary graphs of LTP magnitude.

b, LTP recordings were performed in Syt1,7 double knockdown cells from acute hippocampal slices as described for Fig. 1. BDNF (2 nM) was applied as indicated, following the general protocol of Patterson et al.32 Scale bars below sample EPSCs are 50 pA, 50 ms.

All data are means ± SEM; numbers in bars indicate number of neurons/mice analyzed.