Correction: Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes

Biochem. J. 2017, volume 474, issue 16, pages, 2785–2801; https://doi.org/10.1042/BCJ20170474

In the isotopologue analysis of glycero(phospho)lipids (Figure 5), the authors mistakenly included glycerolipids with only the glycerol moiety labeled (i.e., m+3) in the Glycerol + Fatty Acid labeling groups (Glycl+FA). Fractional enrichment of ¹³C into Glycl+FA of panels B−F should include only those glycerolipids having both the glycerol and fatty acyl chains labeled (e.g. this would include m+5, m+7, m+9, m+11, m+13, m+15, etc.). The corrected figure is presented here. These changes do not change the conclusions drawn from the study and do not require modifications to the text of the manuscript.

Figure 5. Phosphofructokinase regulates glycerolipid biosynthesis.

Stable isotope tracing of phospholipids and triacylglycerols in cardiomyocytes incubated with media containing ¹³C₆-glucose for 18 h: (A) atom-resolved map illustrating the biological and biochemical history of ¹³C incorporation into glycerolipids; fractional enrichment values of ¹³C into: (B) PC; (C) PI; (D) PE; (E) PS; and (F) TAG. Graph represents three replicates per group from one isolation. *^,$P < 0.05, **^,$$P < 0.01, ****P < 0.0001.