Article title: A strategy for L-isoleucine dioxygenase screening and 4-hydroxyisoleucine production by resting cells
Authors: Chenglin Zhang, Jie Ma, Zhixiang Li, Yunlong Liang, Qingyang Xu, Xixian Xie, and Ning Chen
Journal: Bioengineered
DOI: 10.1080/21655979.2017.1361259
The authors regret that some errors occurred in this article.
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(1)
“Asp182” is incorrect and should be replaced by “Asn182” in the article.
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(2)
Table 3 is incorrectly provided and the correct Table 3 is as follows.
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(3)
The E-mail of corresponding author is “ ningch66@hotmail.com”, but not “ ningch66@hotamil.com”.
Table 3.
Primers.
| Primers | Sequence (5’-3’) | Description |
|---|---|---|
| sucA-1 | AAGCATAAAAAAGATGCTTAAGGGATCACGTAACGGCTGACATGGGAATTAG* | Deletion of sucA |
| sucA-2 | TATCTACGCTACTCATTGTGTATCCTTTATTTGAGCGATTGTGTAGGCTGG | |
| sucA-3 | ACGTAGACAAGAGCTCGCAAGTG | Identification for sucA deletion |
| sucA-4 | CGGGTTTTTTATGCCAGGTTG | |
| aceA-1 | AACCACCACATAACTATGGAGCATCTGCACTAACGGCTGACATGGGAATTAG | Deletion of aceA |
| aceA-2 | CGGCCTACAGTCAGCAACGGTTGTTGTTGCTTGAGCGATTGTGTAGGCTGG | |
| aceA-3 | TGGAACAGATCACCACTTCCG | Identification for aceA deletion |
| aceA-4 | ATGATAAGACGCGCAAGCGT | |
| ido-1 | GCCCGGGGGATCCACTAGTTAAGGAAGCTAGATATGAAAATGAGTGGCTTTAGCATAG | Amplification of ido and expressed by pWSK29 |
| ido-2 | GGTGGCGGCCGCTCTAGTTATTTTGTCTCCTTATAAGAAAATGTTACTA | |
| ido-3 | GGATCCATGAAAATGAGTGGCTTTAGCATAG(BamH I) | Amplification of ido and expressed by pET-His |
| ido-4 | AAGCTTTTATTTTGTCTCCTTATAAGAAAATGTTACTA(Hind III) |
Underlined letters indicate the sequence 30 bp upstream or downstream of the gene (or 20 bp upstream or downstream of Xba I recognition site of pWSK29) to be deleted are underlined. Recognition sites are in italics and restriction enzymes are shown in parentheses.
The authors would like to apologize for any inconvenience caused.