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. 2017 Oct 27;8(62):105238–105250. doi: 10.18632/oncotarget.22164

Figure 5. CTHRC1-mediated promotion of invasion and metastasis in vitro and in vivo.

Figure 5

(A) Protein lysates were prepared from cells transduced with lentiviral shnon-target (shTRC2) or shCTHRC1 (sh1997 and sh1999). Membranes were incubated with the indicated antibodies. (B) Wound closure capacity of SH-J1 cells with CTHRC1 knockdown was measured by wound healing assay. Quantitative measurements of wound closure ability are shown (n = 3, mean ± SEM). *P < 0.05. (C) SH-J1-luc cells transduced with lentiviral shnon-target (shTRC2) or shCTHRC1 (sh1997 and sh1999), then 5 × 105 cells via the tail vein to nude mice. Five weeks after tumor implantation, Bioluminescent images demonstrated that CTHRC1- expressing tumor cells spread to the lungs (n = 7 mice/group, representative anterior-posterior images, 30 s exposure time). Quantities are the measured means of photon flux (right panel). Mean ± SEM. ***P < 0.001. Color bar (Counts, Max/Min); SH-J1-luc, 33106/2315; shTRC2, 16669/1107; sh-7, 7194/614; sh-9, 64/58. (D) Tumor-bearing lungs were inflated with India ink to detect tumor nodules. (E) Fifty-five days after tumor implantation, mice were euthanized and their lungs were evaluated for tumor nodules. Each value is mean ± SEM, ***P < 0.001. (F) Immunohistochemical staining of CTHRC1 in metastatic nodules. Scale bars, 100 μm.