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| Gene | Primers (5′—3′) | |
|---|---|---|
| E-cadherin | Forward primer | ATTTTTCCCTCGACACCCGAT |
| Reverse primer | TCCCAGGCGTAGACCAAGA | |
| Vimentin | Forward primer | AGTCCACTGAGTACCGGAGAC |
| Reverse primer | CATTTCACGCATCTGGCGTTC | |
| Agrin | Forward primer | CCGCCAGGAGAATGTCTTCAA |
| Reverse primer | TTTCGTAGGTGACTCCGTCGT | |
| GAPDH | Forward primer | GAGCCACATCGCTCAGAC |
| Reverse primer | CTTCTCATGGTTCACACCC | |
