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. 2017 Nov 20;8(63):106740–106752. doi: 10.18632/oncotarget.22537

Figure 5. CHOP upregulation plays a critical role in OP-A-induced paraptosis-like cell death.

Figure 5

(A) U343, U251N, U251MG and A172 cells were treated with indicated concentration of OP-A for 12 h and Western blotting of CHOP and α-tubulin was performed. (B–E) T98G cells transfected with the non-targeting siRNA (siNT) or CHOP siRNA were further treated with 2 μM OP-A for 16 h. (B) CHOP knockdown was confirmed by western blotting (left). Cellular viability was assessed using calcein-AM and EthD-1 (right). Data represent the means ± SD (n = 7). One-way ANOVA and Bonferroni’s post hoc test. *P < 0.001 vs. untreated control, #P < 0.05 vs. OP-A treatment. (C) Treated cells were observed under the phase-contrast microscope. Bar, 20 µm. (D) YFP-ER cells were transfected with siNT or CHOP siRNA and further treated with 2 μM OP-A for 16 h. Phase-contrast and fluorescence microscopy was performed. (E) Treated cells were subjected to immunocytochemistry of PDI and CHOP. Representative pictures of cells are shown. Bar 20 μm.