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. Author manuscript; available in PMC: 2017 Dec 22.
Published in final edited form as: Biochemistry. 2010 May 4;49(17):3695–3702. doi: 10.1021/bi902211w

Figure 1.

Figure 1

Human myosin IIIA constructs. (A) Schematic diagram of myosin IIIA constructs used in this study. The human myosin IIIA kinase domain construct (huM3AKD) contains Met1–Gly327, a C-terminal c-myc tag, and an octahistidine tag. The human myosin IIIA motor domain construct (huM3AMD) contains Met311–Lys1058 and a C-terminal FLAG tag. (B) SDS–PAGE of purified myosin IIIA constructs. huM3AKD (a) and huM3AMD (b) were expressed in Sf9 cells and purified as described in Experimental Procedures. The purified proteins were analyzed by SDS–PAGE. No CaM band is observed since the huM3AMD has no IQ motifs.