Table 3.
Biofilm formation on polystyrene plates with the four different growth media used at 6 °C and 22 °C by Salmonella strains tested in this study.
| Strains | Media | |||||||
|---|---|---|---|---|---|---|---|---|
| 1/20 TSB | 1/20 Milk | TJ | CMJ | |||||
| St. | 6 °C | 22 °C | 6 °C | 22 °C | 6 °C | 22 °C | 6 °C | 22 °C |
| T1 | 0.112 ± 0.007 a | 0.366 ± 0.089 a,* | 0.092 ± 0.010 a,b | 0.262 ± 0.077 b,* | 0.079 ± 0.002 b | 0.061 ± 0.004 d | 0.083 ± 0.003 b | 0.100 ± 0.015 c |
| T4 | 0.149 ± 0.012 a | 0.395 ± 0.159 a,* | 0.084 ± 0.011 b | 0.139 ± 0.036 b,* | 0.083 ± 0.007 b | 0.059 ± 0.006 d,* | 0.080 ± 0.004 b | 0.088 ± 0.016 c |
| T5 | 0.146 ± 0.045 a | 0.169 ± 0.057 a | 0.099 ± 0.026 b | 0.160 ± 0.089 a | 0.078 ± 0.009 c | 0.080 ± 0.005 c | 0.077 ± 0.005 c | 0.111 ± 0.013 b,* |
| T6 | 0.152 ± 0.012 a | 0.219 ± 0.038 a,* | 0.090 ± 0.009 b | 0.144 ± 0.052 b,* | 0.057 ± 0.005 d | 0.071 ± 0.010 d | 0.076 ± 0.004 c | 0.113 ± 0.018 c,* |
| ET1 | 0.112 ± 0.007 a | 0.461 ± 0.069 a,* | 0.101 ± 0.014 a,b | 0.199 ± 0.032 b,* | 0.082 ± 0.008 b,c | 0.088 ± 0.010 c | 0.079 ± 0.010 c | 0.091 ± 0.015 c,* |
| B2 | 0.232 ± 0.037 a | 0.452 ± 0.042 a,* | 0.108 ± 0.026 b | 0.160 ± 0.033 b,* | 0.080 ± 0.010 c | 0.116 ± 0.012 c,* | 0.085 ± 0.003 c | 0.156 ± 0.022 b |
| N5 | 0.201 ± 0.022 a | 0.424 ± 0.049 a,* | 0.105 ± 0.017 b | 0.191 ± 0.035 b,* | 0.110 ± 0.013 b | 0.081 ± 0.002 c | 0.097 ± 0.025 b | 0.105 ± 0.034 c |
| I4 | 0.076 ± 0.008 a | 0.131 ± 0.025 a,* | 0.104 ± 0.039 a | 0.117 ± 0.061 a | 0.100 ± 0.021 a | 0.118 ± 0.032 a | 0.111 ± 0.039 a | 0.116 ± 0.025 a |
| I5 | 0.158 ± 0.012 a | 0.404 ± 0.135 a,* | 0.104 ± 0.021 b | 0.146 ± 0.021 b | 0.082 ± 0.003 c | 0.125 ± 0.022 b,* | 0.079 ± 0.004 c | 0.085 ± 0.022 c |
| AZ3 | 0.115 ± 0.013 a | 0.142 ± 0.063 a | 0.080 ± 0.010 b | 0.089 ± 0.020 b | 0.083 ± 0.005 b | 0.060 ± 0.009 c,* | 0.079 ± 0.006 b | 0.089 ± 0.019 b |
| AZ6 | 0.131 ± 0.015 a | 0.098 ± 0.011 a,* | 0.087 ± 0.020 b | 0.100 ± 0.018 a | 0.090 ± 0.010 b | 0.067 ± 0.010 b,* | 0.082 ± 0.007 c | 0.097 ± 0.012 a |
| SA2 | 0.642 ± 0.098 a | 0.563 ± 0.034 a | 0.130 ± 0.040 b | 0.300 ± 0.060 b,* | 0.079 ± 0.010 c | 0.081 ± 0.011 d | 0.080 ± 0.020 c | 0.121 ± 0.022 c,* |
| SA3 | 0.433 ± 0.019 a | 0.566 ± 0.061 a,* | 0.124 ± 0.032 b | 0.314 ± 0.039 b,* | 0.081 ± 0.001 c | 0.066 ± 0.009 d,* | 0.077 ± 0.012 c | 0.096 ± 0.009 c,* |
| 0.210 ± 0.161 a | 0.341 ± 0.165 a,* | 0.101 ± 0.020 b | 0.179 ± 0.070 b,* | 0.084 ± 0.015 b | 0.083 ± 0.023 c | 0.081 ± 0.014 b | 0.105 ± 0.022 c,* | |
Different letters in the same row and the same temperature indicate significant differences (p < 0.05). Asterisks indicate significant differences (p < 0.05) between the two temperatures for the same growth medium. Strains code corresponds to those in Table 1. St.: strains; : Average. Tryptic Soy Broth 1/20 at 1/20 (w/w) (1/20 TSB); Milk at 1/20 (w/w) (1/20 Milk); Tomato Juice (TJ); Chicken meat juice (CMJ).