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. 2018 Jan 1;8(1):78–91. doi: 10.7150/thno.21278

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Production and characterization of the human anti-hMUC1 monoclonal antibody. (A) Mice were immunized with PBS or rhMUC1-EC192 plus Lipoplex (O) and sera were analyzed for the presence of rhMUC1-EC192-specific antibody using ELISA. (B) Anti-hMUC1 monoclonal antibody was purified from ascites and analyzed using SDS-PAGE followed by Coomassie blue staining. HC: heavy chain; LC: light chain. (C) Isotype of IgG in the purified antibody fraction was determined using ELISA. (D) Determination of binding affinity for the rhMUC1-EC192 protein using an SPR system. The rhMUC1-EC192 protein was immobilized on a sensor chip, and increasing amounts of antibody were applied. Kinetic parameters are shown in the right panel.