Biodistribution of the anti-hMUC1 monoclonal antibody in T47D cell-derived breast tumor tissue. BALB/C nu/nu mice were subcutaneously injected with T47D cells to induce tumor formation. (A) The mice were injected intravenously with DyLight 755-labeled normal IgG (5 mg/kg) or anti-hMUC1 monoclonal antibody (5 mg/kg), and whole-body fluorescent imaging was performed at 0, 24, and 48 h. (B) The localization of the antibody in dissected mice. (C) Antibody distribution in various organs and tumors. Scale bars, 2.5 cm (A-C). (D) The mice were injected intravenously with the DyLight 488-labeled anti-hMUC1 antibody. Tumor sections were stained with DAPI and evaluated using confocal microscopy. Scale bars, 10 μm (D). The images are representative of data from 3 sets of mice.