Figure 1.

Summary of imaging protocol. A baseline contrast scan was captured prior to the microbubble injection (A). A scan to capture the maximum peak intensity was taken 1 min after the introduction of contrast (B). The bubbles were allowed to circulate and bind for 7 min, at which time a contrast scan was taken (C). Destructive pulses clear the tumor of both bound and freely-flowing bubbles (D), and the level of freely-flowing contrast was obtained 1 min after destruction to allow bubbles to reperfuse the tissue (E). Note: data was only collected at B, C, and E and the solid black line is only an estimate of the intensity over time.