Figure 1.

Downregulation of RHCG in clinical ESCCs. (A) RHCG signal was detected by cDNA microarray in four paired ESCC/nontumor samples (#301, #327, #351 and #363), and one pair of esophageal dysplasia tissue and adjacent normal tissue (#314). (B) RHCG expression in matched nontumor and ESCC tumor samples (n = 45) as detected by qRT-PCR. GAPDH was used as an internal control. The data displayed as average ΔC_t values. Boxes in the box plot contain the values between the 25^th and 75^th percentiles. The lines across the boxes indicate the median. (C) Representative IHC staining of RHCG in ESCC tumor and paired nontumor squamous epithelial tissue (Scale bar = 200 µm). (D) Bar chart summary of the distribution of different RHCG expression levels in nontumor versus tumor for all informative cases on the TMA. (E) Kaplan-Meier survival analysis comparing the overall survival time of ESCC patients with different RHCG expression levels. Statistics: B, paired t test; E, Kaplan-Meier analysis and log-rank test.