Table 1.
The following primers were used in our study.
| Primer Name | Primer Sequence | References |
|---|---|---|
| Igfbp5 (WT)-Forward | 5′-CTCGAGGTCCTCCCCTCGCCCCATCCCATCC-3′ | NCBI Reference Sequence: XM_847792.4 |
| Igfbp5 (WT)-Reverse | 5′-GTTTAAACTAAATGAGATGAAATGAGTGGCGTC-3′ | |
| Igfbp5 (mut)-Forward | 5′-CATTTGAACTGATTTAGT-3′ (5′ phosphorylation) | |
| Igfbp5 (mut)-Forward | 5′-CAGTTCAAATGAGTGGCGTCCT-3′ (5′ phosphorylation) | |
| Igfbp5-Forward (qPCR) | 5′-GACTCCAGCCAGCACCT-3′ | |
| Igfbp5-Reverse (qPCR) | 5′-AGACCTTGCTAGCGATTCCGA-3′ | |
| TP53-Forward | 5′-ACAGTAGTGACGGTCTTGCC-3′ | GenBank: AB020761.1 |
| TP53-Reverse | 5′-CAACCTCGGGTGGCTCATAA-3′ | |
| bcl-2-Forward | 5′-CATGCCAAGAGGGAAACACCAGAA-3′ | [37] |
| bcl-2-Reverse | 5′-GTGCTTTGCATTCTTGGATGAGGG-3′ | |
| bax-Forward | 5′-TTCCGAGTGGCAGCTGAGATGTTT-3′ | |
| bax-Reverse | 5′-TGCTGGCAAAGTAGAAGAGGGCAA-3′ | |
| caspase3-Forward | 5′-TTCATTATTCAGGCCTGCCGAGG-3′ | |
| caspase3-Reverse | 5′-TTCTGACAGGCCATGTCATCCTCA-3′ | |
| caspase8-Forward | 5′-ACAAGGGCATCATCTATGGCTCTGA-3′ | |
| caspase8-Reverse | 5′-CCAGTGAAGTAAGAGGTCAGCTCAT-3′ | |
| caspase9-Forward | 5′-TCAGTGACGTCTGTGTTCAGGAGA-3′ | |
| caspase9-Reverse | 5′-TTGTTGATGATGAGGCAGTAGCCG-3′ | |
| GAPDH-Forward | 5′-TTCCACGGCACAGTCAAG-3′ | [38] |
| GAPDH-Reverse | 5′-ACTCAGCACCAGCATCAC-3′ |
WT: wildtype; mut: mutation; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.