Figure 6. miR-564 regulates signaling pathways downstream of GRB2.

(A) Expression levels of GRB2, E-cadherin, N-cadherin, p-AKT, AKT, vimentin, p-ERK1/2 and ERK1/2 in the miR-NC + vector, miR-NC + GRB2, miR-564 + vector, miR-564 + GRB2, GRB2-scramble and shGRB2 groups were measured by western blot. (B–C) The ratio of each protein to GAPDH. All assays were repeated three times, and the mean values were used for comparisons. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001.