Figure 1. Transfection with TUSC2 reduces PD-L1 expression in non-small cell lung cancer.

H157, H1975, and HCC827 cells were transiently transfected with 4 μg of TUSC2 cDNA for 48 hours and the cells were then harvested and lysed. There were no significant toxicity observed. (A) Phospho-mTOR, mTOR, TUSC2, and β-actin control signals were detected using Western blot analysis. (B) Phospho-S6 ribosomal protein and phospho-p70 S6 kinase, as well as PD-L1, were detected by Western blot analysis. No change was observed in the S6 ribosomal protein, p70 S6 kinase protein. Proteins were quantified using the UN-SCAN-IT automatic digitizing system. Those intensities of the signals of PD-L1 are relative to β-actin.