Figure 5. Combined treatment of BRAF mutant A375 and COLO205 cells with SHR8443 and dabrafenib or trametinib.

(A) A375 and COLO205 cells were incubated with the indicated concentrations of each compound or their combinations for 72 h. Cell viability was determined by SRB assay. CI, combination index. (B) A375 and COLO205 cells were incubated with SHR8443 (100 nM), trametinib (10 nM), dabrafenib (100 nM), or their combinations for 72 h, and analyzed by annexin V-FITC/PI staining and flow cytometry. ^*P < 0.05, versus either single agent treatment. (C) A375 and COLO205 cells were incubated with SHR8443 (100 nM), trametinib (10 nM), dabrafenib (100 nM), or their combinations for 2 h. Whole-cell lysates were analyzed by Western blotting using the indicated antibodies.