Figure 1. Phenotypes of H1975DM, H1975C5 and H1975C6 cells.

(A) Concentration response curves to BEZ235 at 72 h post-treatment. (B) Cell cycle phase distribution following 24h exposure to IC₅₀ concentrations and 10μM BEZ235. (C) Phase contrast images of cells at 20x magnification in migration (wound-healing) assays following 24 h exposure to DMSO and IC₅₀ concentrations or 10 μM BEZ235. (D) Apoptosis levels after 24 h exposure to IC₅₀ concentrations and 10 μM BEZ235, and 0.1μM docetaxel as a positive control. (E) Baseline protein levels of PTEN, and ratios of phosphorylated to total ERK, AKT, S6RP and 4EBP1, determined by western blotting and densitometry. All levels were normalized to GAPDH. (F) Western blots of respective proteins in cells after 24h exposure to DMSO, and IC₅₀ concentrations and 10μM BEZ235. ^* p < 0.05, paired t-test. All experiments were performed in triplicate.